Experience:

2 years required

Skills:

Biology, English

Job type:

Full-time

• Accelerated Development: Our venture studio model streamlines the typically lengthy process of biotech innovation. With access to expert operational, legal, finance, and HR teams, you can bypass common startup hurdles and focus immediately on scientific advancement and strategic growth..
• Integrated Resource Access: You will have direct access to state-of-the-art labs, cutting-edge infrastructure, and specialized development and product teams tailored to your project s unique needs facilitating rapid iteration and optimized drug develop ...
• Collaborative Synergies: Joining our venture studio connects you with a vibrant ecosystem of experienced entrepreneurs, distinguished scientists, and industry leaders. This collaborative environment encourages cross-pollination of ideas, mentoring opportunities, and strategic partnerships, enhancing both innovation and your professional growth..
• Strategic Support and Investment: Beyond initial capital, Great Good provides ongoing strategic guidance and operational support. Our comprehensive back-office resources help manage day-to-day complexities while our investment in your venture ensures you have the means to scale quickly and capture market opportunities..
• Enhanced Commercial Potential: By working under the venture studio umbrella, you not only drive scientific breakthroughs but also elevate the commercial viability of your project. Our model is designed to maximize innovation in drug therapeutics and generate significant market value, positioning your venture for long-term success and impactful industry leadership..
• About the Role.
• We are seeking a motivated and experienced CRISPR Scientist to join our dynamic R&D team and drive the design, execution, and analysis of our gene editing projects. If you are passionate about translating innovative science into transformative medicine, we encourage you to apply..
• Design and execute experiments using various CRISPR-Cas systems (e.g., OPENCRISPR1, Cas9, Cas12a, Base Editors, Prime Editors) for gene editing in mammalian cells, including primary cells and immortalized cell lines. This scope of work is expected to include using machine learning platforms to screen, analyze, synthesize and test enzymes of the Cas and deaminase classes as well as nickase conversion..
• Develop and optimize methods for gRNA design, delivery (e.g., lipofectamine, lipid nanoparticles, electroporation), and assessment of editing efficiency and specificity using PCR and NGS technologies..
• Analyze and interpret complex data sets, including molecular biology assays (qPCR, Western Blot, ELISA), next-generation sequencing (NGS) data for on-target and off-target editing, and functional cell-based assays..
• Culture and manipulation of various cell lines (HEK-293, HeLa, iPSCs, and T cells), with a focus on validating utility of novel complexes to perform precise editing in cells..
• Troubleshoot experimental hurdles and contribute creative solutions to accelerate project timelines. This will include minimizing off-target and bystander editing by correct selection of PAMs and other critical sequences..
• Document experiments in electronic lab notebooks and present results clearly at internal meetings..
• Stay current with the latest scientific literature, technology advancements, and regulatory requirements pertaining to CRISPR and gene therapy..
• Collaborate effectively with interdisciplinary teams including technicians and scientists, in the immunology, cell biology, drug development and bioinformatics fields..
• Required.
• Ph.D. in Molecular Biology, Cell Biology, Genetics, Biochemistry, or a related field..
• Minimum of 2+ years of relevant post-doctoral or industry experience focused on gene editing techniques, specifically CRISPR technology. Experience in Base Editors and human T cells is desired..
• Deep expertise in mammalian cell culture techniques, including transfection and viral transduction..
• Proficiency in molecular biology techniques, including DNA/RNA isolation, PCR/qPCR, cloning, and gel electrophoresis..
• Demonstrated experience with assays used to quantify gene editing outcomes (e.g., T7 Endonuclease I assay, NGS library preparation and analysis)..
• Excellent written and verbal communication skills with the ability to present complex data effectively..
• Preferred.
• Industry experience in a Biotech or Pharmaceutical setting..
• Experience with non-Cas9 CRISPR systems (e.g., Base/Prime Editors, dCas9 fusions) is a strong plus..
• Familiarity with in vivo delivery methods and animal models..
• Experience with high-throughput screening and automation..
• Experience with CAR-T cell development.
• Experience with antiviral therapeutics..
• What We Offer.
• Competitive compensation and equity package in an early-stage, high-growth startup..
• The opportunity to make a significant impact on developing next-generation therapies..
• A collaborative, fast-paced, and intellectually stimulating culture..
• To Apply, please submit your CV and a cover letter detailing your relevant experience to [email protected] or Application Link: https://forms.gle/Ewgtmcgwuv6B5KJ66..